Environmental Engineering Reference
In-Depth Information
as took place for the known dilution series and the nucleic acid concentration
represented by the
C
t value is read from the calibration curve.
Quality control and assurance is uppermost in all phases of PCR method-
ology. Prospective analysts should be aware of the U.S. Environmental
Protection Agency publication “Quality assurance/quality control guidance for
laboratories performing PCR analyses on environmental samples,” available at
http://www.epa.gov/nerlc/cwww/qa qc pcr10 04.pdf.
Advances in molecular methods of detecting and quantifying microorganisms
should be powerful assets to modern environmental epidemiology. The poten-
tial exists for analyzing samples for the presence of suspect pathogens in water
supplies with far greater certainty than can occur by conventional methods.
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