An Advanced Monitoring Platform for Rational Design of Recombinant Processes - Measurement, Monitoring, Modelling, and Control of Bioprocesses - page 65

An Advanced Monitoring Platform
for Rational Design of Recombinant
Processes

G. Striedner and K. Bayer

Abstract Bioprocess engineering is an application-oriented science in an
interdisciplinary environment, and a meaningful combination of different scientific
disciplines is the only way to meet the challenges of bioprocess complexity.
Setting up a reasoned process monitoring platform is the first step in an iterative
procedure aiming at process and systems understanding, being the key to rational
and innovative bioprocess design. This chapter describes a comprehensive process
monitoring platform and how the resulting knowledge is translated into new
strategies in process and/or host cell design.

Keywords Chemometrics Host and process design Prediction Process
monitoring Recombinant protein production

Abbreviations

ANN Artificial neural networks

amu Atomic mass unit

CER Carbon dioxide evolution rate

CRP Catabolite regulation protein

CDM Cell dry mass

cAMP Cyclic adenosine monophosphate

DC Dead cells

EMA European medicines agency

GFP Green fluorescent protein

ppGpp Guanosine tetraphosphate

HCDC High-cell-density cultivation

ICH International conference on harmonisation

IPTG Isopropyl b-D-thiogalactopyranoside

MVDA Multivariate data analysis

MS Mass spectrometry

NIR Near-infrared

G. Striedner ( ) K. Bayer

Department of Biotechnology, University of Natural Resources and Life Sciences,
Muthgasse 18/B, A-1190 Vienna, Austria
e-mail: [e-mail]

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