Biomedical Engineering Reference
In-Depth Information
Fig. 3 Schematic of the dilution setup in an FIA: injection valve 1 is shown in the loop loading
position. When it is switched (by a 608 rotation), the reactor is fed with the contents of the loop
first and then with carrier fluid only (upper time course of c in ). Given that the reactor behaves as
an ideally mixed continuous stirred tank, the concentration at the outlet (equal to that loaded into
injection valve 2) follows the trajectory shown at the bottom for a ratio of loop volume to reactor
volume of 0.02; in other words, a thousand-fold dilution can be achieved after roughly three mean
residence times. It is a matter of timing when injection valve 2 is switched to determine the
desired dilution used further downstream in the FIA
to eliminate gas bubbles, namely repetitive flushing with small volumes of ace-
tonitrile, was applied. Sohn et al. [ 84 ] packed immobilized enzymes into an SIA,
considering further miniaturization on microfluidic chips. In this work, glucose
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