Biomedical Engineering Reference
In-Depth Information
Fig. 1 Heat flow rate profile
for pure aerobic respiratory
growth of K. marxianus
cultivated in glucose-limited
mineral salt medium. Phases:
A—exponential growth,
B—fed-batch growth, and
F—feed start
18
16
F
14
12
B
10
8
6
4
A
2
0
0
2
4
6
8
10
12
Time (h)
Fig. 2 Feedback control of
specific growth rate of
K. marxianus employing
real-time heat flow rate
measurements
0.30
0.25
Setpoint = 0.2 h -1
0.20
0.15
0.10
7
8
9
10
11
12
Culture time (h)
are encouraging and suggest that heat flow calorimetry may be a potential PAT
process analyser in a variety of bioprocess systems.
Very few reports have been published on the use of heat-flow calorimetry using
mammalian bioprocess systems. Real-time monitoring and control of mammalian
bioprocesses is gaining considerable attention in the biopharmaceutical industry to
achieve high yields of monoclonal antibodies (mAbs) and other recombinant
proteins. Mammalian cell growth processes are slow reactions and weakly exo-
thermic compared with microbial anaerobic systems [ 16 ], and may require mod-
ifications to increase instrument sensitivity or to yield a high-resolution signal by
minimizing the heat losses to the environment through insulation and ambient
temperature control. Signal quality could also be improved by employing robust
noise filtering techniques.
 
Search WWH ::




Custom Search