The Choice of Suitable Online Analytical Techniques and Data Processing for Monitoring of Bioprocesses - Measurement, Monitoring, Modelling, and Control of Bioprocesses - page 254

Table 1 Bioprocess monitoring and control applications of heat-flow biocalorimetry
Bioprocess system Key process parameters (CPPs) a Calorimeter

model

Growth metabolism Sensitivity

(mW L -1 )

Reference

Cultivation in mixed
substrate mode

BSC-81 Aerobic B100 Onset of diauxic growth [ 21 ]

Batch cultivation of E.
coli

BSC-81 Aerobic B100 Glucose limitation [ 8 ]

Dual limitation of
substrates

RC1 Aerobic respiration/

aerobic
fermentative

100 Onset of uncoupled respiration [ 19 ]

Controlled fed-batch
cultivation

RC1 Aerobic respiration/

fermentative

100 Sustaining oxidative growth metabolism, high productivity using

repeated fed-batch addition of limiting N-source

[ 27 - 29 ]

Anaerobic growth of
methanogen and
UASB sludge

BioRC1 Anaerobic 5-10 Cell growth monitoring, biomass adaptation, onset of aceticlastic

methanogenesis

[ 23 , 24 ,
30 ]

Investigation of biological
activity of WWT
system

BioRC1 Aerobic/anaerobic/

anoxic

5-10 Real-time biomass activity, toxicity tolerance and nitrification/

denitrification assessment

[ 10 , 20 ]

Fed-batch cultivation of
Bacillus spharecius

BioRC1 Aerobic [10 Substrate consumption rate and feed control [ 11 ]

Quantitative studies on
micro-algal
cultivation

BioRC1 Photoautotrophic 5-10 Real-time characterization of autotrophic, heterotrophic and

mixotrophic growth phases & estimation of photosynthetic
efficiency

[ 25 , 26 ,
31 ]

Large-scale calorimetry of
Bacillus sphaericus

300-L
bioreactor

Aerobic 150-500 Monitoring and control of a Bacillus spaericus culture [ 32 ]

Large-scale calorimetry 100,000-L

bioreactor

Aerobic Real-time monitoring of a S. cerevisiae culture by biocalorimetry

at large-scale

[ 33 ]

(continued)