The Choice of Suitable Online Analytical Techniques and Data Processing for Monitoring of Bioprocesses - Measurement, Monitoring, Modelling, and Control of Bioprocesses - page 249

The Choice of Suitable Online Analytical
Techniques and Data Processing
for Monitoring of Bioprocesses

Ian Marison, Siobhán Hennessy, Róisín Foley, Moira Schuler,
Senthilkumar Sivaprakasam and Brian Freeland

Abstract With increasing pressure from regulatory authorities on industry to
develop processes embracing process analytical technology (PAT) initiatives,
there is a growing demand to establish reliable tools and systems capable of
meeting this need. With regard to monitoring and control of bioprocesses, this
need translates to a search for robust instrumentation capable of monitoring the
critical process parameters in real time. The application of such technologies at all
stages of the process, from the initial R&D phase to process optimisation and
production, enhances process understanding and paves the way for the develop-
ment of control platforms. An examination of the PAT concept and selected tools
(NIR, MIR, Raman, dielectric spectroscopy and calorimetry) are presented here. A
description of each tool is given, with particular emphasis on the nature of the
signal produced and how these relate to measurements of biomass, metabolites and
product. A description of the signal processing that is necessary to gain meaningful
results from the different tools is also given, together with online data reconcili-
ation techniques based on mass and energy balances. Many techniques such as
those based on vibrational spectroscopy are of particular interest, since they are
capable of monitoring several critical process parameters which are typically
controlled in a bioprocess. A window of application for each of the techniques,
when used in the area of bioprocessing, is suggested based on their uses and
inherent limitations.

Keywords Bioprocess control Bioprocess monitoring Calorimetry Critical
process parameters Data reconciliation PAT Spectroscopy

I. Marison ( ) S. Hennessy R. Foley M. Schuler S. Sivaprakasam B. Freeland

Dublin City University, Dublin Ireland
e-mail: [e-mail]

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