Coating of Material Surfaces with Layer-by- Layer Assembled Polyelectrolyte Films - Biological and Biomedical Coatings: Processing and Characterization - page 356

(a) (a)'

(b) (b)'

(c) (c)'

(d) (d)'

(e) (e)'

FIGURE 8.10
Immunochemical and histochemical staining of troponin T (a marker for myotube) (a-e) and alkaline phos-
phatase (a marker for osteoblasts) (a′-e′) of C2C12 on BMP2 loaded films for increasing BMP-2 initial concen-
trations: (a) 0; (b) 0.5 μg/mL; (c) 1 μg/mL; (d) 10 μg/mL, (e) 50 μg/mL (scale bar: 150 μm). (Reproduced with
permission from Crouzier et al., Small , 5, 598-608, 2009. Copyright Wiley-VCH Verlag GmbH & Co. KGaA,
2009.)

osteoblastic activity) depending on the amount of BMP2 loaded in the films (Figure 8.10)
(Crouzier et al. 2009).
A first step in the development of nanomaterials for neural interfaces was provided by
Jan and Kotov (2007), who demonstrated the differentiation of environment-sensitive neu-
ronal stem cells (NSCs), both as neurospheres and single cells, on a (PEI/single-wall carbon