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restrict the up-scaling of breeding materials used
in MABS. In addition, backcrossing was not per-
formed in off-season in this study because of
lower temperatures in the greenhouse. If back-
crossing were to be performed in off-season as
well, the breeding term would be shortened as
described by Chu and colleagues (2011). Until
now, most of the studies on the molecular breed-
ing of peanut have focused on the development of
selection markers and demonstration of their util-
ity. However, it would be important to determine
an ideal balance between the required amounts
of plant material and the markers used under
limited facility and labor conditions. The suc-
cess of MABS is not achieved only by introduc-
ing molecular work in a breeding program. The
entire design of the breeding procedure includ-
ing field and greenhouse management plays an
important role for obtaining maximum results
from MAS.
Chen, Z., Wang, M.L., Barkley, N.A., Pittman, R.N.
(2010) A simple allele-specific PCR assay for detecting
FA D 2 alleles in both A and B genomes of the cultivated
peanut for high-oleate trait selection. PlantMolBiolRep
28:542-548.
Chu, Y., Ramos, L., Holbrook, C.C., Ozias-Akins, P. (2007)
Frequency of a loss-of-function mutation in oleoyl-pc
desaturase ( ahFAD2A ) in the mini-core of the U.S. Peanut
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Chu, Y., Holbrook, C., Ozias-Akins, P. (2009) Two alleles
of ahFAD2B control the high oleic acid trait in cultivated
peanut. Crop Sci 49:2029-2036.
Chu, Y., Wu, C.L., Holbrook, C.C., Tillman, B.L., Person.
G., Ozias-Kins, P. (2011) Marker-assisted selection to
pyramid nematode resistance and the high oleic trait in
peanut. The Plant Genome 4:110-117.
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content. Plant Physiol 151:1030-1040.
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