Biology Reference
In-Depth Information
Fig. 11.4.
Mean O/L ratio of the F
2
seeds of nine genotype
combinations of
ahFAD2A
and
ahFAD2B
loci. 'YI-0311,'
'Hetero,' and 'Nakate' indicate homozygous 'YI-0311,' het-
erozygous, and homozygous 'Nakateyutaka' genotypes on
the loci, respectively. Black line shows standard deviation.
Fig. 11.3.
Fatty acid content in the two parental lines
and the 204 F
2
seeds. Black, white, and gray diagonal line
bars indicate fatty acid content of 'YI-0311,' 'Nakateyutaka,'
mean value of the total 204 F
2
seeds, and mean value of
the selected three F2 seeds for high O/L ration, respectively.
Black lines indicate standard deviation.
tent, except when both loci were derived from
'YI-0311.' This result suggests that there are no
significant differences between the functions of
the two
FA D 2
genes in the peanut plant. Chu
and colleagues (2009) investigated the genetic
mutation of
FA D 2
genes in peanut cultivars bred
in the U.S.A., and discovered the existence of
two types of mutations in
ahFAD2B
linoleic acid (Figure 11.3). The content of
palmitic and stearic acid was low, and the major
fatty acids present in the seed were oleic and
linoleic acids. The O/L ratios of the parental
lines 'Nakateyutaka' and 'YI-0311' were 0.98
±
0.11 and 49.4
4.9, respectively. The O/L ratio
of the F2 lines ranged from 0.84 to 35.2. The
oleic acid content was positively correlated with
the sum of the oleic acid and the linoleic acid
content (r
±
−
transpo-
son insertion and SNP
while a single mutation
was observed in
ahFAD2A
. The identified muta-
tions on
FA D 2
genes in 'YI-0311' were same
as those reported by Chu and colleagues (2009).
This suggested that the diversity in the muta-
tion alleles on the two
FA D 2
genes is limited,
and the markers developed in the present and
previous studies is effective for multiple donor
lines showing high O/L ratios (Chu et al. 2009;
Barkley et al. 2010).
−
0.69), and not significantly corre-
lated with linoleic acid content (r
=
=
0.13) with
a significant level of P
0.05. A significant
association between the O/L ratio and geno-
types on
ahFAD2A
and
ahFAD2B
was identified
by the GMM analysis with an F value
<
1619.7
(Figure 11.4). This result suggested that the
genetic effects of the two loci in relation to the
high O/L ratio were recessive, and a high O/L
was associated with the combination recessive
alleles of both
ahFAD2A
and
ahFAD2B
.
Brunner and colleagues (2001) reported
that the linoleate content in cells expressing
ahFAD2A
was about 40% of that in cells
expressing
ahFAD2B
when they were separately
expressed in yeast (
Saccharomyces cerevisiae
).
In our results, no significant differences were
observed in either O/L ratio or linoleic acid con-
=
Practice of MABS for High O/L in Peanut
As indicated in Figure 11.1, the MABS pro-
gram is currently in progress (2012 January),
and BC cycle 3 has not been completed. In this
section, we trace the progress of the MABS
program from the beginning until BC Cycle2
(Table 11.1). A total of 58 seeds were obtained
Search WWH ::
Custom Search