Marker-Assisted Backcrossing Selection for High O/L Ratio in Cultivated Peanut - Translational Genomics for Crop Breeding: Abiotic Stress, Yield and Quality - page 181

Fig. 11.1. MABS breeding scheme for high O/L ratio in peanut. ' AABB, '' AaBb, '
and ' aabb ' indicate genotypes of individuals on ahFAD2A and ahFAD2B . A and
a show genotypes of 'Nakateyutaka' and 'YI-0311' on ahFAD2A , respectively,
whereas B and b represent genotypes of 'Nakateyutaka' and 'YI-0311' on ahFAD2B ,
respectively. In F 2 ,BC 1 F 2 ,BC 2 F 2 and BC 3 F 2 generations, nine genotype combi-
nations were observed on the two FA D 2 loci: AABB , AaBB , aaBB , AABb , AaBb ,
aaBb , AAbb , Aabb ,and aabb . The expected frequency of ' aabb ' genotype is 1/16,
and plants showing ' aabb ' genotypes were selected for the next BC cycle. 'Self
checking,' 'FAD2,' and 'GW' represent the investigation of genotypes of a DNA
marker to check for existence of seeds developing from self-pollination, ahFAD2A
and ahFAD2B genes, and genome-wide DNA markers.

3 ) and the TaqMan probes (FAM-labeled
5 -CCTCGACCGC A ACG-3 for mutant allele
and VIC-labeled 5 -CCTCGACCGC G ACG-3
for wild-type alleles; Applied Biosystems, USA)
on the 7900HT Fast Real-Time PCR System
(Applied Biosystems, USA). The TaqMan assay

lines and F 2 progenies (Jung et al. 2000;
Lopez et al. 2000; Patel et al. 2004). The
SNP in ahFAD2A was identified by using
the TaqMan assay with the primer pairs (5 -
CCCTTCACTCTTGTCTATTAGTTCCTTAT-
3 and 5 -TGATACCTTTGATTTTGGTTTTGG-