Biomedical Engineering Reference
In-Depth Information
Fig. 3.1.
Schematic drawing of 2D-TLC of DABTH derivatives of amino acids (modified from
C
HOLI
-P
APADOPOULOU
T et al. (1997). In: K
AMP
RM et al. (eds.) Protein structure analysis, p. 146.
Springer, Berlin).
A
alanine,
C
cysteine,
Cm
caboxymethyl cysteine,
D
aspartic acid,
E
glutamic
acid,
F
phenylalanine,
G
glycine,
H
histidine,
I
isoleucine,
K1
α
ε
α
-DABTH-
-DABTC-lysine,
K2
-PTH-
ε
α
ε
α
ε
-DABTC-lysine,
K3
-DABTH-
-PTC-lysine,
Kac1
-DABTZ-N-
-acetyl-lysine (after hydrolysis of
α
ε
α
ε
acetyl group),
Kac2
-DABTH-N-
-acetyl-lysine,
Kform1
-DABTZ-N-
-formyllysine (after hydroly-
ε
ε
ε
sisof formyl group),
Km
N-
-methyl-lysine,
Kd
N-
-dimethyllysine,
Kt
N-
-trimethyllysine,
L
leucine,
α
ε
α
ε
M
methionine,
N
asparagine,
O1
-DABTH-
-DABTC-ornithine,
O2
-PTH-
-DABTC-ornithine,
α
ε
O3
-PTC-ornithine,
POH1
DABTZ-4-hydroxyproline,
POH2
4-hydroxyproline,
POH3
4-
hydroxyproline (after hydrolysis),
Q
glutamine,
R
arginine,
S
serine,
S
-DABTH-
∆
dehydroserine,
Sx
poly-
merized serine,
SD
DABTZ-serine,
Sm
O-methyl-serine,
SmD
DABTZ-O-methyl-serine,
T
threonine,
T
∆
dehydrothreonine,
TO
DABTZ-threonine,
Tx
polymerized threonine,
Tm
O-methyl-threonine,
TmD
DABTZ-O-methyl-threonine,
U
thiourea derivative,
V
valine,
W
tryptophan,
Y
tyrosine,
d
and
e
, referencemarker of DABITC-reaction products of diethylamine and ethanolamine, respec-
tively.
DABITC
4-N,N
dimethylaminoazobenzene-4
-isothiocyanate;
DABTH
diaminoazobenzene-
thiohydantoin,
DABTZ
dimethylaminoazobenzene-thiazolinone,
PITC
phenylisothiocyanate,
PTH
phenylthiohydantoin
