Biomedical Engineering Reference
In-Depth Information
8.10 Ammonium Sulfate Saturation Table
Ammonium sulfate is one of the cheapest and smoothest agents for
precipitation of proteins. It is suitable for protein concentration as
wellasforproteinfractionation.
For description of the amount of ammonium sulfate used for
precipitation mostly the term “% saturation” is used, because at
a given temperature the concentration of a saturated ammonium
sulfate solution is constant. But because the concentration of sat-
urated solution is temperature dependent, the description should
also contain data for temperature of saturated ammonium sulfate
solution used for precipitation as well as for sample solution.
Tables 8.19 (25
◦
C) and 8.20 (0
◦
C) show the amount of solid
ammonium sulfate needed to bring up a solution with lower content
to a higher percentage of saturation.
Table 8.18.
Concentration of ammonium sulfate in saturated solutions
at different temperatures
Temperature
Molarity
Percent
Density
/
/
(
◦
C)
3
)
(moles/l)
(
w
w
)
(
w
v
)
(g/c
0
3.9
41.42
51.48
1.243
10
3.97
42.22
52.52
1.244
20
4.06
43.09
53.65
1.245
25
4.1
43.47
54.12
1.247
30
4.13
43.85
54.59
1.249
References
Dawson RM, Elliot DC, Elliot WH, Jones KM (1986) Data for biochemical
research, 3rd ed. Clarendon Press, Oxford, p 537
