Biomedical Engineering Reference
In-Depth Information
8.7 Spectroscopic Data of Nucleotides
References
Dawson RMC, Elliott DC, Elliott WH, Jones KM (1986) Data for biochemical
research, 3rd ed. Clarendon Press, Oxford
Table 8.15.
Nucleotides: spectroscopic data and molecular mass
λ
max
(nm)
ε
max
ε
260
Nucleotide
M
r
M
r
(stable formulation)
10
−3
×
5
-AMP
259
15.4
15.4
347.2
365.2 (
×
H
2
O)
5
-ADP
259
15.4
15.4
427.2
471.2 (Na
2
salt)
5
-ATP
259
15.4
15.4
507.2
605.2 (
×
3H
2
O, Na
2
salt)
3
,5
-cAMP
×
256
14.5
15.0
329.2
347.2 (
H
2
O)
5
-CMP
271
9.0
7.6
323.2
323.2
5
-CDP
271
9.2
7.5
403.1
469.1 (Na
3
salt)
5
-CTP
271
9.1
7.5
483.1
549.1 (Na
3
salt)
5
-GMP
252
13.7
11.8
363.2
443.2 (
×
H
2
O, Na
2
salt)
5
-GDP
252
13.7
11.8
443.1
460.9 (Li
3
salt)
5
-GTP
252
13.7
11.7
523.2
541.0 (Li
3
salt)
3
,5
-cGMP
252
13.7
345.2
367.2 (Na salt)
5
-dTMP
267
9.6
322.2
398.2 (
×
3H
2
O, Na salt)
5
-dTDP
267
9.6
403.4
475.2 (
×
3H
2
O, Li
3
salt)
5
-dTTP
267
9.6
482.2
624.2 (
×
3H
2
O, Na
4
salt)
5
-UMP
262
10.0
9.9
324.2
368.2 (Na
2
salt)
5
-UDP
262
10.0
9.9
404.1
470.1 (Na
3
salt)
5
-UTP
262
10.0
9.9
484.1
550.1 (Na
3
salt)
5
-UDP-
544.3
610.3 (Na
3
salt)
glucose
NAD
260
18.0
18.0
663.4
705.4 (
×
2H
2
O, Li salt)
NADH
259
16.9
16.9
745.4
833.4 (Na
4
salt)
339
6.22
NADP
260
18.0
18.0
743.4
787.4 (Na
2
salt)
NADPH
259
16.9
16.9
745.4
833.4 (Na
4
salt)
339
6.2
FAD
263
38.0
37.0
785.6
807.6 (Na salt)
8.8 Detergents (“Surfactants”)
References
Bjerrum OF, Larsen KP, Wilken M (1983) In: Tscheche H (ed.) Modern
methods in protein chemistry. Springer, Berlin, p 79
Brito RMM, Vaz WLC (1986) Anal Biochem 152:250
Helenius A, Simons K (1975) Biochim Biophys Acta 415:29
Lichtenberg D, Robson RJ, Dennis EA (1983) Biochim Biophys Acta 737:285
Neugebauer JM (1990) Meth Enzymol 182:239
