Biomedical Engineering Reference
In-Depth Information
Suspend the sediment in 10 ml of Soln. C and centrifuge at 4
◦
C
with 1500 rpm for 5 min.Repeatthisstepuntilthelymphocytesare
free from thrombocytes. Dilute the lymphocyte concentrate with
about 20 ml Soln. D for further experiments.
For determination of yield, mix 10
µ
l of the cell suspension with
µ
10
l Soln. E and count using an Neubauer ruling hemocytometer.
