Biomedical Engineering Reference
In-Depth Information
Table 5.4.
Concentration, density, and refractive index of aqueous den-
sity gradient solutions (for sucrose see Table 8.17)
Density gradient medium
Conc.
Molarity
Density
Refract.
ρ
n
2
D
(% w/v)
(g/ml)
CsCl in water (25
◦
C)
a,b
10
0.64
1.079
1.3405
20
1.39
1.174
1.3498
30
2.28
1.286
1.3607
40
3.37
1.42
1.3735
50
4.70
1.482
1.3885
60
6.36
1.785
1.4072
Saturated
7.4
1.91
1.4185
Cs
2
SO
4
in water (25
◦
C)
c
10
0.3
1.086
1.3438
20
0.66
1.19
1.3414
30
1.09
1.317
1.3607
40
1.62
1.469
1.3718
50
2.27
1.644
1.3846
Saturated
3.56
2.01
20
d
Percoll in 0.25 M sucrose
1.054
40
1.078
60
1.102
80
1.125
Stock
1.149
solution
Percoll in 0.15 M NaCl
20
1.027
40
1.051
60
1.075
80
1.098
Stock
1.122
solution
10
f
Metrizamide in water
0.127
1.025
1.3483
(20
◦
C)
e
20
0.253
1.106
1.3646
30
0.38
1.161
1.3809
40
0.507
1.216
1.3971
50
0.633
1.271
1.4133
60
0.76
1.326
1.4295
70
0.887
1.381
1.4458
Ficoll in water (20
◦
C)
10
1.032
1.3469
15
1.05
1.3545
20
1.069
1.3625
25
1.09
1.3713
30
1.103
1.3801
35
1.121
1.3893
40
1.155
1.3986
50
1.199
1.4171
Nycodenz
g
0
0
1.030
1.3450
5
0.061
1.052
1.3512
10
0.122
1.073
1.3575
15
0.183
1.095
1.3637
