Biomedical Engineering Reference
In-Depth Information
Fig. 5.3.
rpm-g graph for fixed-angel Eppendorf tube rotor (Eppendorf
5415 centrifuge; 24
×
1.5ml)
Important!
Rotors, buckets and centrifuge tubes are undergoing
enhanced mechanical stress. Handle with care, avoid damage, and
check rotors, buckets, bottles, and tubes for any sign of damage
prior to running. Balance tubes in diametrically opposed position
carefully. The solutions within the tubes should not only have the
same volume and weight, but especially in the case of high-speed
centrifugation, the same density too. If tubes are placed in threefold
geometry, i.e., if a rotor has six positions, for instance, the three
filled tubes placed at positions 1, 3, and 5, all tubes must have the
same weight and volume. Clean rotors, buckets, and tubes carefully
after running.
References
Osterman LA (1984) Methods in protein and nucleic acid research, vol. 1,
Springer, Berlin
Graham J (2001) Biological centrifugation. Bios, Oxford
5.2 Differential Centrifugation
Differential centrifugation is the simplest form of centrifugation:
A mixed particle population is subjected to centrifugations with
