Biomedical Engineering Reference
In-Depth Information
4 Immunochemical Protocols
The protocols given in this chapter are selected examples of the uni-
verse of modern immunology. The demands of a biochemical lab
gave the background of this selection; therefore, procedures which
afford specialized knowledge, such as, for example, production of
monoclonal antibodies, are omitted, and the reader is referred to
the respective literature.
As with other biochemical methods, the immunochemical pro-
tocols are improved, but modifications are possible and sometimes
desirable.
References
Caponi L, Migliorini P (eds.) (1999) Antibody usage in the lab. Springer,
Berlin
Crowther JR (2001) ELISA - Guidebook. Humana Press, Totowa, N.J.
Harlow E, Lane D (1988) Antibodies. A laboratory manual. Cold Spring
Harbor Laboratory
Hudson L, Hay F (1989) Practical immunology, 3rd ed., Blackwell, Oxford
Kerr MA, Thorpe R (eds.) (1994) Immunochemistry Labfax. Academic
Press, Oxford
Manson MM (ed.) (1992) Immunochemical protocols (Methods in molec-
ular biology, vol. 10). Humana Press, Totowa N.J.
Mohr P, Holtzhauer M, Kaiser G (1992) Immunosorption techniques: fun-
damentals and applications. Akademie Verlag, Berlin
Peters JH, Baumgarten H (eds.) (1992) Monoclonal antibodies. Springer,
Berlin
Phillips TM, Dickens BF (2000) Affinity and immunoaffinity purification
protocols. Eaton Publishing, Natick
Schade R, Behn I, Erhard M, Hlinak A, Staak C (eds.) (2001) Chicken egg
yolk antibodies, production and application. Springer, Berlin
Wild D (2005) The immunoassay handbook. Elsevier, Oxford
4.1 Conjugation of Haptens (Peptides)
to Carrier Proteins
Low-molar mass molecules, such as, for example, chemically syn-
thesized peptides, are in most cases not able to induce antibodies in
animals; therefore, these molecules have to be covalently coupled
