Evolution and Genetic Engineering - Bioprocess Engineering: Kinetics, Biosystems, Sustainability and Reactor Design - page 712

Foreign DNA
Region of interest

Gene for
antibiotic
resistance

Plasmid

EcoRI EcoRI

EcoRI

EcoRI

EcoRI

DNA
purification

Sticky ends

Hybridization
and DNA ligase

Bacterial
chromosome

Culture

Bacterium cell

Recombinant
DNA

DNA insertion

Cloning Only bacteria containing

recombinant DNA grow

Clones

Bacteria platted on
medium with antibiotics

FIGURE 14.6 Method of producing genetically altered DNA.

FIGURE 14.7 Procedure of cloning DNA.