Fed-Batch Cultivation - Bioprocess Engineering: Kinetics, Biosystems, Sustainability and Reactor Design - page 662

CHAPTER
13

Fe d-Batch Cultivati on

OUTLINE

13.1. Design Equations 667 13.7. Parameters to Be Controlled

or Monitored During Fed-Batch
Operations

13.1.1. Overall Mass Balance in
the Reactor 667 687

13.1.2. Mass Balance of the
Substrate in the Reactor

13.7.1. Calorimetry 687

668 13.7.2. Specific Growth Rate 688

13.1.3. Mass Balance on the Cell
Biomass

13.7.3. Substrate (Carbon and
Nitrogen Source) 669 688

13.1.4. Mass Balance on
Extracellular Products

13.7.4. By-product concentration 688

670 13.7.5. Inductive, Enhancer or

Enrichment Components 13.1.5. Energy Balance in the

Reactor

689

670 13.7.6. Respiratory Quotient 689

13.7.7. General Feeding Mode 690

13.2. Ideal Isothermal Fed-Batch
Reactors

13.7.8. Proton Production 690

671

13.7.9. Fluorescence 691

13.3. Isothermal Pseudo-Steady State
Fed-Batch Growth

13.8. Parameters to Start and Finish
the Feed and Stop the Fed-Batch
Fermentation

676

13.4. Advantages and Disadvantages
of Fed-Batch Operations

691

684

13.9. Summary 691

13.5. Considerations in Implementing
Fed-Batch Operations

Problems 692

686

13.6. Examples of Fed-Batch Use in
Industry 686

The fed-batch technique was originally devised by yeast producers in the early 1900s to
regulate the growth in batch culture of Saccharomyces cerevisiae . It was observed that in the
presence of high concentrations of malt, an undesired by-product, ethanol, was produced,