How Cells Grow - Bioprocess Engineering: Kinetics, Biosystems, Sustainability and Reactor Design - page 601

Reading Materials

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PROBLEMS

11.1. A simple, batch fermentation of an aerobic bacterium growing on methanol gave the
results shown in Table P11.1 . Calculate:
(a) Maximum growth rate ( m max )
(b) Yield on substrate (YF X/S )
(c) Mass doubling time (t d )
(d) Saturation constant (K S )
(e) Specific growth rate (

m net )att ¼ 10 h

TABLE P11.1

Time, h X , g/L S , g/L Time, h X , g/L S , g/L

0 0.20 9.23 12 3.20 4.60

2 0.21 9.21 14 5.60 0.92

4 0.31 9.07 16 6.15 0.08

8 0.98 8.03 18 6.20 0

10 1.77 6.80

11.2. The data in Table P11.2 were obtained for Pyrodictium occultum at 98 C. Run 1 was
carried out in the absence of yeast extract and run 2 with yeast extract. Both runs
initially contained Na 2 S. The vol % of the growth product H 2 S collected above the
broth was reported as a function of time as shown in Table P11.2 .

(a) What is the lag time with and without the yeast extract?
(b) What is the difference in the maximum specific growth rates, m max , of the bacteria

with and without the yeast extract?