Biomedical Engineering Reference
In-Depth Information
TABLE 8.4
Methods of Covalent Binding of Enzymes to Supports
Supports with
OH end group
a) Using cyanogen bromide
HC OH
HC O
HC O NH Protein
+ H
2
N Protein
+ CNBr
C NH
HC OH
HC O
HC OH
b) Using S-triazine derivatives
+ H
2
N Protein
N
N
N
OH +
Cl
Cl
O
Cl
O
NH Protein
N
N
N
N
N
N
R
R
R
Supports with
e
NH
2
end group
a) By diazotization
+ Protein
+ Protein
+ NaNO
2
HCl
+ NaNO
2
HCl
-
-
+
+
N
N
N Protein
N Protein
NH
2
NH
2
N
2
Cl
N
2
Cl
b) Using glutaraldehyde
+ H
2
N Protein
NH
2
+ HCO (CH
2
)
3
CHO
N CH (CH
2
)
3
CHO
N CH (CH
2
)
3
CH N Protein
Supports with
e
COOH end group
a) via azide derivative
+ CH OH
H
+
+ H
2
NNH
2
+ NaNO
2
+ H
2
N Protein
3
OCH
2
CONH Protein
OCH
2
COOH
OCH
2
COOCH
3
OCH
2
CONHNH
2
OCH
2
CON
3
HCl
b) Using a carbodiimide
N R
1
C
N R
O
C O
HN R
1
C
N R
O
C
NH Protein +
HNR
1
O C
HNR
+ H
2
N Protein
COOH +
Supports containing anhydrides
CH
2
CH C
CH
2
CH C
O
O
+ H
2
N Protein
NH Protein
O
CH C
CH C
OH
O
O
CH
2
CH
2
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