The Role of Oxidative Stress in the Response of Endothelial Cells to Metals - Biologically Responsive Biomaterials for Tissue Engineering - page 74

Fig. 4.4 Fig. 4.4 The phenotype of HDMEC grown on PS and Ti6Al4V alloy. HDMEC were
cultured on PS ( A-F ) and Ti6Al4V ( G-L ) alloy for 72 h, untreated ( A-C, G-I ) or treated ( D-F, J-L )
with 0.5 mM H2O2 for 24h (H2O2 was added 48 h after seeding of the cells on the materials).
Eventually the cells were fixed and scanning electron microscopical images were taken (scale bar:
A, D, G, J - 200 µm; B, E, H, K - 100 µm; C, F, I, L - 50 µm).
in HDMEC on PS prevent changes in metabolic activity after H 2 O 2 treatment, while
Ti6Al4V itself and H 2 O 2 induce cumulative effects which result in reduced adaptive
potential of the cells.
The phenotype of endothelial cells on Ti6Al4V alloy was visualised with SEM
72 h after seeding the cells on the material and compared to the phenotype of
endothelial cells grown on PS (Fig. 4.4 ). While optically the amount of cells seemed
to be similar on both materials, higher magnification images (200× and 500×) indi-
cated the differences in the shape and distribution of HDMEC on PS and Ti6Al4V.
Thus, HDMEC grown on PS had an almost uniform spindle-like form lying roughly
parallel and forming an almost confluent cell layer. HDMEC on Ti6Al4V alloy, in
contrast, had diverse cell shapes with some cells being more rounded and some
more elongated than the cells on PS. Cell monolayers on Ti6Al4V alloy had gaps
between the cells and lacked the ordered alignment of HDMEC on PS with the cells
growing in different directions.