Phase Imaging in Plant Cells and Tissues - Biomedical Optical Phase Microscopy and Nanoscopy - page 60

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Modulator

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Objective

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Polarizer P2

Polarizer P1

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Figure 4.8
HMC microscope and its operating concept.

adaptive optics element. The optimization of the image quality of the sample is based on
the shape calibration of DMM using an algorithm to mitigate aberration issue. This
experiment produced results that illustrated the capability of single aberration correction in
enhancing the image quality over the conventional wide-field fluorescence microscopy.
Figure 4.9 shows an example of using adaptive optics with a single plane illumination
microscope on Arabidopsis root for a fluorescent case from work led by John Girkin of
Durham University. Such microscopy method is identical to the one performed in Ref. [20] .

4.8 Interferometric Microscopy

The principle of the SLIM in conjunction with a Zeiss manufactured “Axio Observer Z1”
phase contrast microscope is shown in Figure 4.10 while imaging neurons grown in vitro
(taken from Ref. [21] ). A halogen lamp is used as the light source of the microscope.
The specimen on the stage can be pinpointed by the incident lights with the aid of the