Phase Imaging in Plant Cells and Tissues - Biomedical Optical Phase Microscopy and Nanoscopy - page 57

Dark
DIC image
Light
Polarizer
Nomarski
prism
Interference
fringes
Objective
Transparent
specimen
Stage
Substage
condenser
Interference
fringes
Nomarski
prism
Direction of
propagation
Polarizer
From light source
Figure 4.5
DIC light microscopy.
visualized by the human eye, the image of the transparent specimen can be produced using
the phase information of two polarizations. The separation between the two beams is less
than the resolution of the object. By introducing the interference through the recombination
of these dual polarized lights using the second Nomarski prism, the relative phase
difference will be formed, hence enabling visualization for the detector. Figure 4.5
illustrates the process of how the image of transparent specimen can be produced.