Biomedical Engineering Reference
In-Depth Information
(A)
(B)
(C)
Figure 3.3
A two “window” cell preparation for filming. (A) Preparation seen from the side to show the raised
ridges of both sides at the ends of the aluminum support slide. (B) Preparation as seen from the top.
The left “window” is an assembled cell preparation for which the top and bottom coverslips are held
on only with silicone grease. The right “window” is an assembled preparation for which a thin bead
of VALAP is used to strengthen the seating of the cell bearing coverslip. Also the coverslip is marked
with a felt tipped pen to indicate the orientation of the preparation for its removal and replacement
on the stage of the microscope. (C) Enlarged image of the portion of the preparation enclosed by
the dotted line in panel B. This enlargement provides better views of the VALAP bead at the margin
of the coverslip and the pen line inked on the coverslip. These two “window” preparations are well
suited for use with a dry objective with sufficient working distance to clear the VALAP bead mounted
on a microscope with a motorized stage to allow filming of multiple fields in both windows. With
two preparations on the same slide, one can image cells subjected to two experimental conditions or
control plus experimental samples. For immersion objectives with short working distances, one
should use only one “window” of the two or use a support slide with only one “window.”
coordinately maximize the volume of the medium while maintaining optical properties that
allow K¨hler illumination with standard condensers. The ridges going across the end of the
slide on both the top and the bottom surfaces provide clearance for the top and the bottom
coverslip so that they do not drag on the stage. These ridges are put on both top and bottom
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