Biomedical Engineering Reference
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demonstrated their capabilities for optical sectioning imaging with mesoscopic resolution
and metrology with nanometer-level axial localization precision. In particular, we showed
that FPM can be realized in the Fourier domain and is useful for outlining in a single shot
(i.e., without scanning) fluorescence gradients in semitransparent samples. Importantly, our
recent report on image formation in fluorescence coherence-gated imaging suggested that
the coherence gating mechanism may provide a unique approach for the rejection of out-of-
focus fluorescence in thick scattering samples
[47]
. In addition, we discussed the usefulness
of FPM for the precise localization of individual fluorescent emitters in all 3D. In closing,
the potential next steps in the development of FPM would include explorations of its ability
to track dynamical processes as well as its extension to other types of luminescence.
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