Nano-Holographic Interferometry for In-Vivo Observations - Biomedical Optical Phase Microscopy and Nanoscopy - page 369

(B)

1.2

1.1

1

0.9

0.8

y = 25.981 R p -0.7311

2 = 0.9994

R

0.7

0.6

0.5

0.4

0.3

0.2

0.1

0

0 50 100 150 200 250 300 350 400

Radius of the nanosphere (nm)

450 500 550 600 650 700 750

Figure 17.18
Continued.

with the numerically computed WG wavelengths. The graph shown in Figure 17.18B which
is the ratio of equatorial wavelength to radius is plotted versus the radius; this seems to
provide a correlation with a better sensitivity. Once again the correlation was very good.

17.10 Conclusions

New versions of FTH and FTHI were introduced. The basic idea behind these new versions
is the self-illumination process of the observed objects that are at the same time light
sources and imaged objects. The self-luminosity of the observed objects is caused by
electromagnetic resonance. This effect is similar to the self-luminosity of quantum dot
particles, and like in the case of quantum dots, the emitted light frequencies depend on the
size of the nano-object (in this particular case, NaCl nanocrystals) as well as on the
electronic structure of the object material. The observed frequencies correspond to the band
of peak frequencies of NaCl [27] . The presence of a diffracting element in the optical
circuit generates a large amount of k vectors that provide a wide spectrum of exciting
frequencies. This aspect is discussed to some length in Ref. [23] including supporting
evidence.

Another basis of the developed methodologies, supported by the experimental evidence of
the obtained results, is the fact that the self-luminous wavefronts generate propagating
waves that go through the whole process of image formation beyond the restrictions