Biomedical Engineering Reference
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Bright region
where orders
overlap
Nanocrystal
Sh ift Δ x
CCD plane
S hift Δ x
4
Order -1
Order +1
Zero order
Microscopic
Focal plane
3
Focal plane
1
1
0
0
1
0
1
0
2
Spherical relay
Lens
1
1
0
0
Prismatic
nano-crystal
Microscopic slide
1
Figure 17.8
Schematic representation of the optical system leading to the formation of lens hologram: (1)
Prismatic nano-object of dielectric material; (2) wavefronts entering and emerging from the
polystyrene microsphere acting as a relay lens; (3) wavefronts arriving at the focal plane of
the spherical lens; (4) wavefronts arriving at the image plane of the CCD. The simulation of the
overlapping of orders 0,
1, and
1 in the image plane of the CCD is also shown [10] .
1
2
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