Polarization and Spectral Interferometric Techniques for Quantitative Phase Microscopy - Biomedical Optical Phase Microscopy and Nanoscopy - page 297

capable of monitoring selected points with extremely high resolution, with the help of a
broadband light source.
Additional wavelengths also open the possibility for new system functions by exploiting
spectral diversity. We have introduced a multichannel multiplexing scheme, which is
conveniently based on commercial color cameras, for simultaneous recording of
holographic images using multiple wavelengths to remove ambiguities in phase unwrapping
and extend the range of phase measurements. We also used the technique to acquire
coregistered label-free phase maps of a cell and its corresponding fluorescence signature,
providing molecular information to complement the highly resolved structural information.
A highly desired feature of this approach is the single-detector, common-path
coregistration, which minimizes motion artifacts and maximizes imaging speed with
reduced system cost.
While the first three techniques are focused on the measurement of optical/physical
properties of a sample, specifically its OPL, the NLDS technique integrates optical
spectroscopy into spectral-domain QPM, providing quantitative information regarding the
sample's chemical composition and therefore introduces the potential for molecular
specificity.
These are just a few examples that demonstrate the power of polarization and spectral
techniques for extending the abilities of traditional QPM. Since both polarization and
wavelength are among the most fundamental properties of light and can be manipulated,
modified, and detected by numerous optical components and systems, we expect that efforts
in this direction will continue to grow and that a multitude of novel techniques will further
enhance performance and functionality for QPM.
Acknowledgments
Grant support was provided by the National Institutes of Health (National Cancer Institute R01CA138594) and
the National Science Foundation (CBET-0651622 and MRI-1039562).
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