Holographic Motility Contrast Imaging of Live Tissues - Biomedical Optical Phase Microscopy and Nanoscopy - page 207

(A)

Reference
beam

Target

OP1

L1 L2 CCD

L3

IP1

PBS

OP2

BS

FP1

FP2

Probe
beam

(B)

OP2

OP1 FP1 IP1 FP2

L1 L2 L3

c

a

D

b

Field of
view

Figure 11.1
OCI system. (A) The layout of the lenses, beamsplitters, and CCD camera in an off-axis
holography configuration. (B) The apertures, Fourier and image planes of the OCI system. OP1,
first optical plane; FP1, first Fourier plane; IP1 and OP2, first image plane and second object
plane; and FP2, second Fourier plane coincident on the face of the CCD camera.

for the wavelength λ . The subtended solid angle for the collecting lens L1 is approximately

4 /100. The aperture diameter at FP1 is set equal to a , and the speckle diameter at the first

image plane IP1 is

π

4
π

f 2 a

d IP1 (11.2)

5

c

The magnification at the first image plane IP1 is

f 2
f 1

M 5 (11.3)

and the size of the sample image on the image plane before the third lens (FT lens) is
equal to MD samp . Therefore, the speckle diameter at the second Fourier plane FP2 (after
lens 3) is

f 0 λ
D samp

4
π

f 3 λ
MD samp 5

4
π

d FP2 (11.4)

5

c

where the effective focal length of the three-lens configuration is

f 1 f 3
f 2

f 0 5 (11.5)