Biomedical Engineering Reference
In-Depth Information
The setup, as clearly depicted in the above figures, can be defined as a quasi-Fourier digital
holographic configuration as the point reference beam lies, approximately, on the same
plane as the object plane
[54]
. In the numerical reconstr
uc
tion process, the spherical
reference wave and the chirp function are, respectively,
R
5
exp
f
i
2
2
π=ðλrÞ
U
½ðξρ
ξ
Þ
1
ðηρ
η
Þ
g
2
2
and
W
5
exp
f
2
i
π=ðλdÞ
U
½ðξρ
ξ
Þ
1
ðηρ
η
Þ
g;
where
d
is the reconstruction distance,
r
is
the curvature radius of the beam, and
directions
(i.e., hologram plane). The fast Fourier transform (FFT) calculated for
d
5
r
1
δ
ρ
ξ
and
ρ
η
are the pixel sizes in
ξ
and
η
δ{r
(i.e., the case of quasi-Fourier configuration), can be approximated to the FFT calculated
, with
3
rad
10 µm
5µm
0
(A)
(B)
Figure 10.5
(A) Reconstructed amplitude and (B) phase of the complex wavefield diffracted from a latex particle.
3
rad
0
(A)
(B)
3
rad
0
(C)
(D)
Figure 10.6
Quantitative phase contrast maps of a mouse cell during the different phases.
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