Biomedical Engineering Reference
In-Depth Information
Fig. 25 a
Schematic illustration of the formation and working mechanism of P-RFRTs. Adapted
from Zhen et al., with permission from American Chemical Society. Copyright 2013
Hft is also conjugated with PEG molecules which enhanced their in vivo stabil-
ity. The results shows the capacity of this system in the field of protein-based
theranostic (Fantechi et al. 2014). More recently, Falvo et al. (2014) developed a
tumor targeted antibody-drug conjugate (ADC) by conjugating an average of three
molecules of monoclonal antibody (mAb) Ep1 specific to the human melanoma-
specific antigen CSPG4 and around 50 CDDP molecules to a single ferritin-based
NPs. The conjugate specifically bind to a CSPG4
+
melanoma cell line, but not to
a CSPG4-breast carcinoma cell line as compare to alone Hft-Pt, which has higher
preference for later.
A new therapeutic approach has been developed by using a ferritin nanocages
as versatile antigen delivery nanoplatforms by genetically introducing a antigenic
peptides, OT-1 (SIINFEKL) or OT-2 (ISQAVHAAHAEINEAGR) which are
derived from ovalbumin, either onto the exterior surface or into the interior cavity
of the protein cage for dendritic cell (DC)-based vaccine development. The deliv-
ered antigenic peptides successfully provoked antigen specific CD8
+
or CD4
+
T
cell proliferations both in vitro and in vivo that lead to the differentiation of CD8
+
and CD4
+
T cells into effective cytotoxic T cells and functional CD4
+
Th1 and
Th2 cells producing IFN-
ʳ
/IL-2 and IL-10/IL-13 cytokines, respectively (Han
et al. 2014).
A novel NIR dye (new cyanine green, IR820)-loaded ferritin (DFRT) nanoc-
ages based “chameleon” theranostic platform having strong absorbance in the
NIR region for simultaneous fluorescence/photoacoustic/photothermal multimodal
imaging-guided enhanced PTT both in vitro and in vivo. Moreover under a low
laser power density 100 % in vivo tumor elimination is reported on intravenous
injection of DFRT (Huang et al. 2014). A novel highly specific and sensitive tumor
