Biology Reference
In-Depth Information
X
Low
High
Hybrid
H/L
H/L
H/L
H/L
H/L
H/L
H/L
Mapping Population
Colony Phenotypes
High-Strain
Sister Queens
Figure 6.2.
Males derived from a high/low hybrid queen were used to construct
the linkage map in Figure 6.1. h e same males were then used to instrumentally
inseminate high-strain queens and hence became fathers of colonies that were
used for the QTL map.
about 6 to 10 weeks, all the workers in their colonies were their of spring.
We then evaluated 38 colonies for the amount of stored pollen and as-
signed the colony phenotype to the father of the colony. We assumed that
because the queens were all super sisters derived from a breeding pro-
gram with strong selection, they were fairly uniform for genes that
af ected the pollen-hoarding trait. h ey shared more than 87 percent of
their genes in common. h erefore, the majority of the heritable varia-
tion we observed among colonies was due to the ef ect of the genetic
contributions of the fathers.
