Biomedical Engineering Reference
In-Depth Information
35. Schmidt, M., Schwarzwaelder, K., Bartholomae, C. et al . (2007) High-resolution insertion-site
analysis by linear amplification-mediated PCR (LAM-PCR). Nature Methods , 4 , 1051-1057.
36. Killary, A.M. and Lott, S.T. (1996) Production of microcell hybrids. Methods , 9 , 3-11.
37. Dieken, E.S. and Fournier, R.E.K. (1996) Homologous modification of human chromosomal genes
in chicken B-cell x human microcell hybrids. Methods , 9 , 56-63. This is a reference manuscript
in which we can find a detailed protocol for the microcell fusion.
38. Dieken, E.S., Epner, E.M., Fiering, S. et al . (1996) Efficient modification of human chromoso-
mal alleles using recombination-proficient chicken/human microcell hybrids. Nature Genetics , 12 ,
174-182. This manuscript represents one of the most appealing examples of chromosome transfer
and describes a targeted modification of the human β -globin locus by homologous recombination
using chicken pre-B cell lines, DT40.
39. Buerstedde, J.M. and Takeda, S. (1991) Increased ratio of targeted to random integration after
transfection of chicken B cell lines. Cell , 67 , 179-188.
40. Epner, E., Reik, A., Cimbora, D. et al . (1998) The beta-globin LCR is not necessary for an open
chromatin structure or developmentally regulated transcription of the native mouse beta-globin
locus. Molecular Cell , 2 , 447-455.
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