RNA Amplification Strategies: Toward Single-Cell Sensitivity - Genomics: Essential Methods - page 93

Table 5.2 Comparison of various amplification methodologies.

Vendor Kit Amp type Amp Total RNA Time

method input (ng) required

Affymetrix One Cycle Sample IVT 1000 − 15 000 1 d

Two Cycle Sample IVT 10-100 2 d

Agilent Quick Amp Sample IVT 200 6 h

Ambion MessageAmp ™ II Sample IVT 0.1-20 2 d

MessageAmp ™ III Sample IVT 35-500 6 h

MessageAmp ™ Sample IVT 20-500 6 h

Premier

Clontech Super SMART ™ Sample PCR 2-150 3 h

Enzo BioArray

Single-Round v2

Sample IVT 500-5000 1 d

Epicenter TargetAmp ™

1-Round Biotin
105

Sample IVT 25-500 6 h

TargetAmp 2

Round Biotin v3

™ Sample IVT 0.05-0.1 2 d

TargetAmp ™

aRNA 2.0

Sample IVT 0.01-0.5 2 d

Genisphere 3DNA ™

FlashTag

Signal Dendrimer 500-3000 1 h

™

RampUp ™ Sample Tandem IVT 1 2 d

SenseAmp™ Sample IVT 25-250 1 d

Illumina TotalPrep ™ RNA Sample IVT 50 1 d

RiboAmp ® HS
Plus

Molecular
Devices

Sample IVT 0.1-0.5 1d

Ovation ® V2 NuGen Sample RiboSPIA 5-100 4 h

WT-Ovation ® Sample RiboSPIA 5-50 4 h

WT-Ovation ® Pico Sample RiboSPIA 0.5-50 6 h

Sigma-Aldrich Transplex™ Sample PCR 5-50 4 h

System
Biosciences

Full Spectrum ™ Sample PCR 20 3 h

PerkinElmer MICROMAX™

TSA™

Signal Tyramide

deposition

500-1000 2 h

University Health
Network

Iscove et al .,
Global-RT-PCR

Sample PCR 0.01-0.1 5 h