Biology Reference
In-Depth Information
Table 1 (continued)
Enzyme
family
-
Older
designations
Protein and gene
identification
Number References
Q53I60
Tc00.1047053506697.20
TcrPDEC-S d
-
TcPDE4
Q4ZHU6
n.a.
n.a.
Alonso et al.
( 2006 )
-
LmjPDEC
-
Q4FXD4
LmjF29.2680
29
-
-
LinPDEC
-
A4IaX6
LinJ29.2990
29
-
-
LbrPDEC
-
A4HHS5
LbrM29.2730
29
-
-
-
-
-
-
-
PDED
TbrPDED
-
Q57V52
Tb927.3.3340
3
-
-
TcrPDED-P
-
Q4E664
Tc00.1047053508153.260
36 P
-
-
TcrPDED-S
-
Q4D9B7
Tc00.1047053510323.50
36 S
-
-
LmjPDED
-
Q4FXF8
LmjF29.2440
29
-
-
LinPDED
-
A4I4V8
LinJ29.2800
29
-
-
LbrPDED
-
A4HHP3
LbrM29.2460
29
-
Column 5: number of the chromosome on which the gene is located
Tbr: Trypanosoma brucei , Tcr: Trypanosoma cruzi , Lmj: Leishmania major , Lin: L. infantum ,
Lbr: L. braziliensis , EMBL: UniProtKB/TrEMBL accession number, TriTryp: TriTryp database
gene nomenclature; n.a.: data not available
a Genomic locus in Trytryp database incorrectly assembled
b GAF-A domain exchanged with the GAF-A domain of TbrPDEB1 in all strains derived from
Lister 427
c Pseudogene with internal stop codon
d Possibly an allelic variant of Q53160
PDEAs of all three organisms is that they can complement PDE-deficient yeast
strains, indicating that they are active in the yeast cell, but that no biochemically
measurable enzyme activity could be detected in yeast cell lysates. This is in
surprising contrast to findings with the other PDEs (see below).
PDEB . In all kinetoplastids, two isoforms of this enzyme are coded for by two
tandemly arranged genes, PDEB1 and PDEB2. These most likely represent an
ancient gene duplication that predates the divergence of the kinetoplastids. In
T. brucei , both genes are expressed at similar levels in both bloodstream and
procyclic forms. The respective polypeptides consist of 930 (TbrPDEB1) and
925 (TbrPDEB2) amino acids and contain two GAF domains in the N-terminal
region (D 234 -E 554 in TbrPDEB1), followed by a PDE catalytic domain (V 586 -R 908
in TbrPDEB1). The GAF and catalytic domains are highly conserved between
TbrPDEB1 and TbrPDEB2 (96.3 and 88.3% amino acid identity, respectively). In
an ancestor of a series of a popular laboratory strain of T. brucei (the Lister 427
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