Biology Reference
In-Depth Information
Table 2 Substrate specificity of the PDE catalytic domains
a
Enzyme
cAMP
cGMP
Specificity
(k
cat
/K
M
)
cAMP
/
K
M
k
cat
k
cat
/K
M
K
M
k
cat
k
cat
/K
M
(s
1
)
(s
1
M
1
)
(s
1
)
(s
1
M
1
)
(k
cat
/K
M
)
cGMP
(
m
M)
(
m
M)
10
3
10
3
PDE4D2(1-507)
1.5
0.2
3.9
0.3
2.7
0.4
1.0.
0.1
5.2
0.8
5.3
0.8
509
10
3
10
3
PDE7A1(130-482)
0.2
0.03
1.6
0.2
7.9
0.1
3.9
0.7
6.8
1.3
1.8
0.5
4,400
10
3
10
3
PDE8A1(480-820)
1.8
0.1
4.0
0.1
2.2
0.1
1.6
0.1
1.6
0.2
1.0
0.1
2,200
10
3
10
3
PDE5A1(535-875)
3.0
0.8
2.1
0.8
0.69
0.07
5.1
1.3
1.3
0.3
0.27
0.08
1/391
10
3
10
3
PDE9A2(181-506)
0.2
0.02
0.05
0.01
0.28
0.05
0.14
0.02
1.0
0.2
7.0
0.07
1/25,000
5.9
0.6
10
3
10
3
PDE10A2(448-789)
0.056
0.005
0.33
0.02
4.4
0.3
1.2
0.1
0.27
0.01
22
PDE4D 0.5-4.0
PDE5A 2.0
PDE9A 0.07-0.39
a
PDE4D2 is the full-length protein. References are as follows: PDE4D2 and PDE7A1 (Wang et al.
2005
), PDE8A1 (Wang et al.
2008
; Yan et al.
2008
),
PDE5A1 (Wang et al.
2006
); PDE9A2 (Huai et al.
2004
), PDE10A2 (Wang et al.
2007a
); full length PDE4D (Bolger et al.
1997
; Mehats et al.
2002
); PDE5A
(Turko et al.
1998
); PDE9A (Soderling et al.
1998
; Fisher et al.
1998
)
