Agriculture Reference
In-Depth Information
sugar beet shreds and capacity to supplement the industrial
Trichoderma reesei
enzyme
cocktail for further application in wheat straw saccharification [26]. In the investigation of
fungal secretomes, one more microorganism
Sclerotium rolfsii
was identified as the most
versatile producer of a mix of pectinolytic, hemicellulolytic, cellulolytic and polymer-
degrading redox enzymes when grown on a sugar beet shreds medium [27].
Because pectin content of sugar beet waste is high it is often used as cheap medium
component for production of pectinolytic enzymes [28, 29, 30, 31, 32]. Production of alkaline
pectin lyase by
Bacillus clausii
[31] on this substrate could be very interesting from the aspect
of
establishing sustainable agriculture. Namely, this enzyme
shows potential as a biological
control agent against several plant diseases
thus eliciting disease resistance.
Fungal pectin lyase with the same potential for biological control was produced by solid
state cultivation of
Aspergillus niger
on sugar beet waste material
[29]. Microbial conversion
of sugar beet extraction waste into pectinolytic activities by
Polyporus squamosus
was
studied in optimization experiments. It was found that combination of concentration of
phosphate and pH of media was essential for high secretion of enzymes [33]. Sugar beet
shreds have also been converted into extracellular alkaline pectinase by
Bacillus gibsoni
[30]
and into exo-pectinase by
Bacillus pumilus
[32].
Figure 1.
Time course of produced (a) biomass, (b) endo-p and (c) exo-p activity during the cultivation
of
P
.
squamosus
in (
) homogeneous medium and in (
) the top and (
) the bottom phase of ATPS
medium, containing dry sugar extraction waste [28].
