Environmental Engineering Reference
In-Depth Information
Solution:
Proportioning factor = 3825 mL ÷ (9 samples × 4.25 MGD) = 100
Volu me 8a.m. = 3.88 × 100 = 388 mL
Volu me 9a.m. = 4.10 × 100 = 410 mL
Volu me 10a.m. = 5.05 × 100 = 505 mL
Volu me 11a.m. = 5.25 × 100 = 525 mL
Volu me 12noon = 3.80 × 100 = 380 mL
Volu me 1p.m. = 3.65 × 100 = 365 mL
Volu me 2p.m. = 3.20 × 100 = 320 mL
Volu me 3p.m. = 3.45 × 100 = 345 mL
Volu me 4p.m. = 4.10 × 100 = 410 mL
BIOCHEMICAL OXYGEN DEMAND CALCULATIONS
Biochemical oxygen demand (BOD 5 ) measures the amount of organic matter that
can be biologically oxidized under controlled conditions (5 days at 20°C in the dark).
Several criteria determine which BOD 5 dilutions should be used for calculating test
results. Consult a laboratory testing reference manual (such as Standard Methods )
for this information. Two basic calculations are used for BOD 5 . The irst is used for
samples that have not been seeded, and the second must be used whenever BOD 5
samples are seeded. Both methods are introduced and examples are provided below.
Bod 5 (u nseeded )
[
] × 300
DO
(mg/L)
DO
(mg/
L)
mL
start
final
BOD unseeded)
=
(16.5)
5
Sample volume(mL)
Example 16.6
Problem: A BOD 5 test has been completed. Bottle 1 of the test had dissolved oxygen
(DO) of 7.1 mg/L at the start of the test. After 5 days, bottle 1 had a DO of 2.9 mg/L.
Bottle 1 contained 120 mg/L of sample. Determine the unseeded BOD 5 .
Solution:
(
) × 300
7.1 mg/L
2.9 mg/L
mL
BOD unseeded)
=
=
10.5 mg/L
5
1
20 mL
Bod 5 (s eeded )
If the BOD 5 sample has been exposed to conditions that could reduce the number of
healthy, active organisms, the sample must be seeded with organisms. Seeding requires
the use of a correction factor to remove the BOD 5 contribution of the seed material:
 
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