Agriculture Reference
In-Depth Information
3.2. Discriminating and Classifying Structural Differences
between Raw Green-Type (CDC Strike) and Raw Yellow-Type
(CDC Meadow)
The implications of the multivariate analyses are that with a pool of
spectra we only need to analyze an unknown spectrum of interest to discover
whether technologically processed plant-based food or feed samples are
similar to the untreated ones (Doiron et al., 2009a,b).
In this study, the Ward‟s
algorithm method was used in CLA analysis without any prior
parameterization of the spectral data (original anti-symmetric and symmetric
CH
3
and CH
2
spectral data) in the two regions (ca. 3001-2799 cm
-1
; ca. 1771-
1714 cm
-1
). PCA analysis was applied to identify the main sources of variation
in the same two regions to identify features that differed between these
cultivars and treatments.
Figures 4 (c,d) clear shows that two classes can be distinguished in the
region of ca. 1771-1714 cm
-1
based on 1
st
PC between raw Green-Type (CDC
Strike) and raw Yellow-Type (CDC Meadow), indicating that the structure
associated with lipid carbonyl C=O ester differ between the green-type and
yellow-type of pea varieties. Figure 4 (a,b) also shows that separate classes in
the region of ca. 3001-2799 cm
-1
can be distinguished based on 2
nd
PC
between the green-type and yellow-type of pea varieties indicating that the
structures associated with lipid related anti-symmetric and symmetric CH
3
and
CH
2
differ between the green-type and yellow-type of pea varieties.
3.
C
ONCLUSION
,
I
MPLICATIONS AND
F
UTURE
R
ESEARCH
In conclusion, there were no difference in spectral profiles of lipid
biopolymer structure conformation between raw green-type (cv. CDC Strike)
and yellow-type (cv. CDC Meadow) of CDC pea varieties. The implication of
this study is that the internal structural of feed and food could be detected on a
molecular basis within intact tissue without destroying the inherent structure
which usually damaged by wet-chemical lab processing and digestion.
I: Raw Green-Type (CDC Strike) vs. Raw-Yellow Type (CDC Meadow Peas) in anti-
symmetric and symmetric CH
3
and CH
2
region
