Agriculture Reference
In-Depth Information
2.3. Antisymmetric and Symmetric CH
3
and CH
2
and Carbonyl
C=O Ester Molecular Spectral Profiles of Green-Type and
Yellow-Type of CDC Peas (
Pisum sativum
)
Tables 1-3 shows molecular structure spectral features of CH functional
groups in terms of the peak height of CH3-antisymmetric (CH3as), CH2-
antisymmetric (CH2as), CH3-symmetric (CH3s), CH2-symmetric (CH2s),
carbonyl C=O ester and their ratios of yellow type and green type of CDC
peas. The results indicated there were no significant differences between the
green-type (cv. CDC Strike) and yellow-type (cv. CDC Meadow) of CDC peas
in spectral peak height of CH
3
-antisymmetric (CH
3
as), CH
2
-antisymmetric
(CH
2
as), the ratio CH
3
-antisymmetric (CH
3
as) to CH
2
-antisymmetric (CH
2
as)
(Table 1) and spectral peak height of CH
3
-symmetric (CH
3
s), CH
2
-symmetric
(CH
2
s), the ratio CH
3
-symmetric (CH
3
s) to CH
2
-symmetric (CH
2
s) (Table 2),
but significant differences in both spectral peak height and area intensity of
carbonyl C=O ester at 1745 cm
-1
(Table 3). The CDC Strike (green-type) had
higher peak intensity in carbonyl C=O ester, indicating structure conformation
at 1745 cm-1 differes (Table 3). There were no significant difference in the
ratio of carbonyl C=O ester peak area to total CH (CH3 and CH2) peak area.
Table 1. Molecular structure spectral features of CH functional groups in
terms of the peak height of CH
3
-antisymmetric (CH
3
as), CH
2
-
antisymmetric (CH
2
as) and their ratios of yellow type and green type of
CDC peas
Item
Peas Type
Green-Type
CDC Strike
Yellow-type
CDC Meadow
SEM
1
Peak height of CH
3
-antisymmetric (CH
3
as) and CH
2
-antisymmetric (CH
2
as)
2
C
H
3
as
0.0093
0.0089
0.00037
0.0194
0.0181
0.00068
C
H
2
as
Ratio CH
3
-antisymmetric (CH
3
as) to CH
2
-antisymmetric (CH
2
as)
Ratio of CH
3
as to CH
2
as 0.4849 0.4909 0.00603
1
SEM = pooled standard error of means; Means with the different letter within the same row are
significantly different (P < 0.05).
2
Peak centers for the CH
3
-antisymmetric, CH
2
-antisymmetric were ca. 2957 cm
-1
, 2926 cm
-1
,
respectively and baseline ca. 3021-2800 cm
-1
for the all CH groups.