Agriculture Reference
In-Depth Information
also enhanced phosphorelay of the proteins in the soluble fraction (Hasunuma
et al., 2012).
Estimation of Chlorophyll Concentrations in the Wild-Type
and the Mutants,
R3-1
and
R3-2
Under strong sunlight, the SPAD values of leaflets from the top of stem
(1
st
leaf), second leaf and third leaf were measured. SPAD values indicate
relative concentrations of chlorophyll in the leaflet cells.
Figure 2a. Proteins in soluble fractions and the membrane fractions of leaves under
strong sunlight were labeled for 15 sec by 10
-9
M [
γ
-
32
P]ATP on ice, and the proteins
were subjected to SDS-PAGE fractionation. The autoradiography presented was
exposed for 3 days.
