Agriculture Reference
In-Depth Information
vegetables than fruits. When botanical classification was undertaken, those in
the highest tertile of leguminosae (peas, dried beans, and string beans),
solanaceae (tomatoes and peppers), rosaceae (peach, plums, nectarines, apples,
pears and strawberries), and umbelliferae (carrots) had decreased risk of head
and neck cancer, but no significant association was detected for nine other
botanical groups. Hence consumption of fruits and vegetables is associated
with a diminished risk of head and neck cancer (Freedman et al., 2008).
A
NTI
-P
ROLIFERATIVE
E
FFECT OF
B
OWMAN
-B
IRK
I
SOINHIBITOR FROM
P
EA ON
HT29
C
OLON
C
ANCER
C
ELLS
Bowman-Birk inhibitors (BBI) are stable under acidic conditions of the
stomach and resistant to gastric and intestinal proteases. Sufficient quantities
arrive at the large intestine in active form to exhibit anti-carcinogenic and anti-
inflammatory actions. Recombinant pea BBI (rTI1B) inhibited the
proliferation of human colorectal adenocarcinoma HT29 cells
in vitro
(IC50 =
31μM). It inhibited both trypsin and chymotrypsin, with nanomolar Ki values,
whereas the related mutant had no effect on the serine proteases and colon
cancer cell growth. The growth of non-malignant colonic fibroblast CCD-
18Co cells was not affected by either recombinant protein (Clemente et al.,
2012).
P
EA
L
ECTIN
S
UPPRESSES
G
ROWTH OF
E
HRLICH
A
SCITES
C
ARCINOMA
C
ELLS
I
N
V
ITRO
AND
I
N
V
IVO
IN
M
ICE
The pea produces a lectin (Ye and Ng, 2001; Kabir et al., 2013) which
exerts a growth retarding action on Ehrlich ascites carcinoma cells in vitro as
well as in mice by triggering apoptosis and G2/M cell cycle arrest (Kabir et al.,
2013).
R
IBOSOME
I
NACTIVATING
P
ROTEINS FROM
P
EA
Two ribosome inactivating proteins which inhibit protein synthesis in the
cell-free rabbit reticulocyte lysate system have been isolated from pea (Lam et
