Agriculture Reference
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symbiotic association between rhizobium and pea and bean plants. Root
nodule extensins, referred to as arabinogalactanprotein-extensin (AGPE), are
major constituents of the infection thread matrix. In symbiotically defective
mutants of pea demonstrating abnormal growth of infection threads, the AGPE
epitope is enriched in the extracellular matrix around infected host cells in the
central infected tissue of the nodule and also occurs in the lumen of
Rhizobium-induced infection threads, implying a mis-targetting of AGPE
resulting from defective growth of the infection threads. Mutants in gene
sym33 manifest diminished MAC265 labeling. Labeling is undetectable in
infection threads and droplets, in contrast to wild-type nodules, indicating a
compositional change in the infection thread matrix of sym33 mutants which
may correlate with the lack of endocytosis of rhizobia into the legume
cytoplasm (Tsyganova et al., 2009).
F ERRITIN
Ferritin from pea seed is composed of 26.5-kDa and 28-kDa subunits with
remarkable amino acid sequence homology and yet distinct, indicating that the
ferritin is encoded by at least two genes. The data indicate that pea seed
ferritin has two distinct types of ferroxidase centers found in the 26.5-kDa and
28-kDa subunits, respectively, different from all known ferritins.
Spectrophotometric titration and stopped flow data revealed that 48 Fe(II) ions
can be bound and oxidized by oxygen at the ferroxidase sites, showing that all
ferroxidase sites play a role in fast Fe(II) oxidation. Nevertheless, in contrast
to H and L subunits in equine spleen ferritin (HoSF), the 26.5-kDa and 28-kDa
subunits do not collaborate in iron turnover into the inner cavity of pea seed
ferritin (Li et al., 2009).
D EHYDRIN
An improved method for purification of from soy dehydrin involved
acidic extraction of soy whey was followed by chromatography on copper
charged Chelating Sepharose Big Beads, intermediate hydrophobic interaction
chromatography on Source 15 PHE, and affinity chromatography on blue
Sepharose. The procedure can be adopted for pea dehydrin (Herzer et al.,
2003).
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