Agriculture Reference
In-Depth Information
C
HANNEL
-F
ORMING
P
ROTEIN IN
C
HLOROPLASTS
Tic110 is a channel-forming protein with six transmembrane helices with
a role in the import of proteins produced in the cytosol occurring at the inner
chloroplast envelope. Two N-terminal hydrophobic transmembrane helices
serve as a signal for the localization of the protein to the membrane. The
remaining amphipathic transmembrane helices are found in residues 92-959.
Calcium ions exert a marked effect on channel activity
in vitro
. The channel-
forming protein possesses a redox-active disulfide which potentially can
interact with stromal thioredoxin (Balsera et al., 2009).
O
UTER
E
NVELOPE
M
EMBRANE
P
ROTEIN
16
(OEP16)
OF
P
EA
C
HLOROPLASTS
The protein, a cation-selective channel protein, has been overexpressed in
Escherichia coli .
in the form of inclusion bodies, purified and then folded with
detergent on a Ni-NTA affinity column. CD spectroscopy studies and ¹
⁵
N-
HSQC NMR spectra revealed that the protein is a folded protein composed of
α-helices (Ni da et al., 2011).
C
ELL
W
ALL
F
IBRONECTIN
-L
IKE
A
DHESION
P
ROTEIN
Proteins immunologically related to human fibronectin and showing
affinity for gelatin and heparin are found in the cell walls of epicotyls of
germinating peas under salt stress at 3-4 times higher concentrations than in
unstressed epicotyls. Plant cell wall proteins facilitate better cell anchorage to
the cell wall during salt stress or water deficit and are cell adhesion as well as
signaling molecules (Pellenc et al., 2004).
A
RABINOGALACTANPROTEIN
-E
XTENSIN
(AGPE)
G
LYCOPROTEINS
The root nodule interface between the leguminous host cell and the
microbial symbiont is pivotal for development and differentiation in the
