Agriculture Reference
In-Depth Information
V
ICILIN
Alkaline extraction of pea flour and salt fractionation under acid
conditions have yielded two vicilin fractions, designated as vicilin 1 degrees
and vicilin 2 degrees after purification. Convicilin (a band at approximately 70
kDa) is the third pea globulin found in vicilin 2 degrees (O'Kane et al., 2004).
L
ECTINS
Fluorescein isothiocyanate (FITC)-labelled mannose-specific lectins from
two pea varieties (PG-3 and LFP-48) exert a more pronounced stimulation in
homologous
Rhizobium leguminosarum
strains binding reaction although
reaction with heterologous strains of
Rhizobium
spp has also been observed
indicating that specificity is not very high (Bajaj et al., 2001).
A mannose- and glucose-binding lectin from sugar snap legumes is
unadsorbed on Affi-gel blue gel and Q-Sepharose and adsorbed on SP-
Toyopearl. The lectin
shows striking homology in N-terminal sequences of its
alpha- and beta-subunit to lectin from
Pisum sativum
L. var. Feltham
.
The
only difference lied in the 19th N-terminal residue of the beta-subunit(Ye and
Ng, 2001). Glucose/mannose
binding
Pisum arvense
lectin is composed of two
distinct polypeptide chains: alpha (Mr. 5591 Da) and beta (19986 Da) (Cavada
et al., 2003).
M
ITOCHONDRIAL
T
YPE
II
P
EROXIREDOXIN
A type II peroxiredoxin from
Pisum sativum
(estimated molecular weight
18.6 kDa) has been cloned into the pET3d vector and overexpressed in
E. coli.
The recombinant protein has been purified and crystallized by. X-ray data
revealed that, with the use of hanging-drop vapour-diffusion technique, the
protein crystallized in space group P1, with unit-cell parameters a = 61.88, b =
66.40, c = 77.23 angstroms, alpha = 102.90, beta = 104.40, gamma = 99.07
degrees (Barranco-Medina et al., 2006).
