Agriculture Reference
In-Depth Information
The availability of sizeable amounts of purified PsGA2OX1 facilitates
mechanistic and structural studies (Lester et al., 2005).
C
ARBONIC
A
NHYDRASE
Recombinant pea beta-carbonic anhydrase was purified and crystallized.
Crystals belong to the orthorhombic space group C222, with unit-cell
parameters a = 136.3, b = 142.5, c = 201.4 A,alpha = beta = gamma = 90
degrees. Crystals typically diffracted anisotropically,with a maximal resolution
of 2.0 A in the strongest direction (Kimber et al., 2000).
P
HOTOSYSTEM
II
P
ROTEINS WITH
C
ARBONIC
A
NHYDRASE
A
CTIVITY
Hydrophilic proteins of the photosystem II oxygen-evolving complex
from pea leaves possess a molecular mass of 18 kDa (protein PsbQ), 24 kDa
(protein PsbP), and 33 kDa (protein PsbO).They all exhibit Mn2+dependent
carbonic anhydrase activity (Shitov et al., 2009).
C1
S
UBGROUP
M
ITOGEN
-A
CTIVATED
P
ROTEIN
K
INASE
(MAPK)
MMAPK cascades are important in plant growth and development and in
responses to abiotic and biotic stress. They are classified into four major
groups (A-D) based on their sequence homology. Abundant information about
MAPKs in groups A and B exists but information about the C group is
meager. PsMPK2 is expressed in reproductive as well as vegetative organs.
Expression of pea MPK2 in Arabidopsis thaliana disclosed that mechanical
injury and other stress agents as hydrogen peroxide, jasmonic acid and abscisic
acid elevate the enzyme activity.Hence C1 subgroup MAPKs may play a role
in the stress response (Ortiz-Masia et al., 2008)
