Agriculture Reference
In-Depth Information
G
LUTATHIONE
R
EDUCTASE
The 56-kDa glutathione reductase isozyme with an isoelectric point of 5.4
has been purified from pea leaf peroxisomes. A multiple targeting of
glutathione reductase to chloroplasts, mitochondria and peroxisomes, has been
observed depending on the organelle metabolism under the prevailing growth
conditions.
Peroxisomal glutathione reductase plays a role in abiotic stress conditions,
such as abundant light, darkness, high temperatures, low temperatures,
wounding, and cadmium toxicity, (Romero-Puertas et al., 2006).
A
MINOALDEHYDE
D
EHYDROGENASE
Aminoaldehydes are toxic polyamine breakdown products. Pea
aminoaldehyde dehydrogenase was overexpressed in
E.coli
, purified and
crystallized using the hanging-drop method. Crystals belong to the monoclinic
space group P2(1),with unit-cell parameters a = 86.4, b = 216.6, c = 205.4 A,
beta = 98.1 degrees (Tylichová et al., 2008).
NADP+-L
INKED
I
SOCITRATE
D
EHYDROGENASE
A
homotetrameric 146-kDa NADP+-linked isocitrate dehydrogenase
manifesting optimum pH at 7.5-8 and inactivation at 45-50 degrees C has been
isolated from germinating pea seeds. Maximum enzyme activity is dependent
on presence of Mn2+ and Mg2+ ions (3.75 mM). Activity is partially restored
by addition of Ca2+, Co2+, Cd2+, and Zn2+ ions after demetallation by
dialysis against EDTA and buffer (Srivastava et al., 2001).
C-6
O
XIDASES
C-6 oxidation genes regulate brassinosteroid activation, which involves
the C-6 oxidation of 6-deoxocastasterone to castasterone (CS) and in some
cases further conversion of castasterone to brassinolide (BL).
However, unlike Arabidopsis and tomato C-6 oxidases which further
convert
6-deoxocastasterone to
castasterone
and then
castasterone to
