Tufa-forming biofilms of German karstwater streams: microorganisms, exopolymers, hydrochemistry and calcification - Tufas and Speleothems: Unravelling the Microbial and Physical Controls - page 95

Fig. 8. Maximum likelihood phylogenetic analysis of pennate raphid diatoms from tufa biofilms of the Westerh¨ fer
Bach site WB5 (shown in bold) using 49 complete 18S rDNA sequences (1700 bp long; 559 variable/351 informative
sites). For details of cloning, sequencing and phylogenetic tree analysis see Appendix.

Table 3. Diatom species recovered from tufa stromatolite biofilms at the sampling site WB5, their next closest
relatives from the NCBI GenBank sequence data base and their phenetic sequence similarities

Diatom species Closest relative sequence Phenetic sequence similarity

to closest relative [%]

Achnanthidium minutissimum c Achnanthidium minutissimum AM502032 99.1

Achnanthidium sp. Pauliella toeniata AY485528 98.9

Amphora pediculus Amphora pediculus AM501960 99.1

cf. Amphora* Phaeodactylum tricornutum EF140622 97.8

Gomphonema sp. A Gomphonema micropus AM501964 99.5

Gomphonema sp. B Gomphonema productum AM501993 96.5

Navicula sp. A Navicula cryptotenella AM502029 98.8

Navicula sp. B Navicula radiosa AM501972 98.3

Navicula tripunctata Navicula tripunctata AM502028 99.8

Navicula veneta* Navicula veneta AM501975 99.8

Nitzschia palea c Nitzschia palea AJ867003 99.8

Pinnularia sp.* Pinnularia rupestris AJ867027 98.5

Surirella brebissonii c Surirella brebissonii AJ867029 99.4

*species that were recovered only from the WB5 site.
c species also studied by cultures.