Biomedical Engineering Reference
In-Depth Information
Figure 1
. Range of possible sentences entailed by a word-block construction scheme.
Figure 2
. Scaling of library diversity with increasing sentence length (block count) and in-
creasing number of available words within each block.
constraints all affect the choices of words in the lexicon. Word design can be
maximized by careful design of words, the lexicon, and database elements, as
well as experimental tuning of annealing conditions (e.g., temperature-ramp
rate, pH, and buffer and salt concentrations). A useful tool in this task is the
computer simulations of DNA hybridization, known as BIND, developed by
Hartemink et al. (30).
3.6. Additional Tagging Methods for the DNA Strands
Various sophisticated tagging techniques have been developed by the bio-
technology industry for expression analysis and differential expression analysis..
